Hi Jaewon, I'm using ML version 2.2.31 with eyelink 1000 plus through Ethernet connection. My task duration is around 30 seconds. So it result in over 30000 samples per trial in AnalogData.Eye. Since my task was running on an old PC, the ITI was exceed desired duration (usually around 8 seconds). I'm wondering is that possible to reduce ML sampling rate in eye signal when uby hs230808 - Questions and Answers